A new class of high-contrast Fe(II) selective fluorescent probes based on spirocyclized scaffolds for visualization of intracellular labile iron delivered by transferrin.

A reactivity-based probe of the intracellular labile ferrous iron pool

Improved methods for studying intracellular reactive Fe(II) are of significant interest for studies of iron metabolism and disease-relevant changes in iron homeostasis. Here we describe a highly selective reactivity-based probe in which a Fenton-type reaction with intracellular labile Fe(II) leads to unmasking of the aminonucleoside puromycin. Puromycin leaves a permanent and dose-dependent mar...

Design and applications of methods for fluorescence detection of iron in biological systems.

Fluorescence metalosensors provide a means to detect iron in biological systems that is versatile, economical, sensitive and of a high-throughput nature. They rely on relatively high-affinity iron-binding carriers conjugated to highly fluorescent probes that undergo quenching after metal complexation. Metal specificity is determined by probes containing either an iron-binding moiety of high aff...

Selective Speciation of Ferric Iron by a New Heterocyclic Ligand

The synthesis and analytical applications of the new heterocyclic ligand are described. The complexation reaction of the ligand with some cations was studied in aqueous methanol at room temperature using the spectrophotometric method. Results revealed that only the absorption spectra of the Fe (III)–ligand complex show a high redshift of the absorption maximum in the aqueous methanol soluti...

An amine/imine functionalized microporous MOF as a new fluorescent probe exhibiting selective sensing of Fe3+ and Al3+ over mixed metal ions

Nowadays metal-organic frameworks with multiple luminescent centers are very fascinating as multifunctional luminescent material because of their luminescence properties, which could be systematically tuned by deliberate use of organic ligands and metal ions. In this research, we explored a microporous mixed-ligand MOF for highly selective and sensitive detection of metal ions. A two-fold inter...

A universal fluorogenic switch for Fe(ii) ion based on N-oxide chemistry permits the visualization of intracellular redox equilibrium shift towards labile iron in hypoxic tumor cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc05457a Click here for additional data file.